Figure 1
From: Small interfering RNA against CD86 during allergen challenge blocks experimental allergic asthma
Silencing CD86 expression by transfection with CD86 small interfering RNA (siRNA) on BMDCs. BMDCs were cultured in the presence of GM-CSF for 7 days. The DCs were transfected with 100nM CD86 siRNA and non-specific (control) siRNA by GeneSilencer®. Twenty-four hours after gene silencing, DCs were activated with LPS for 18 hours. Cells were stained with FITC-conjugated anti-CD11c mAb and biotinylated anti-CD86 mAb followed by phycoerithrin (PE)-conjugated streptavidin. CD86 expression on CD11c-positive cells was assessed by flow cytometry. Histograms represent the number of cells at various fluorescent intensities. MFI data was expressed as the mean ± SEM of 4 mice. * indicates statistically significant (P <0.05) difference between the indicated two groups.