Table 1 Glycolytic key candidate genes primers and primer sequences used for amplification using real-time PCR
From: Up-regulation of hexokinase1 in the right ventricle of monocrotaline induced pulmonary hypertension
primer | Primer sequence | PCR products | |
|---|---|---|---|
Forward primer (5’-3’) | Reverse primer (5’-3’) | location size (bp) | |
HK1 | ctgggcttcaccttctcattt | tcgcaagtgggttcttcatac | 227 |
HK2 | gcagcttctggaggttaagaga | gtctcctttctctgtgccatct | 136 |
HK3 | gtctgatgatgccttgatggt | cctggagaagtggggatgtag | 184 |
PFK | ggatgtctgggaaaatcaaaga | gctcaaaatctgtctggtcctt | 141 |
PK | tggtgacagaagtggagcac | gcacaaaggaggcaaagatg | 159 |
PDHα1 | ccatctcatcactgcctatcg | agcctcctcttcgtcctgtta | 98 |
PDHα2 | tgtggctctcgcctgtaaata | aaatacctgcccttggttagc | 88 |
PDHβ | tagttgcccattcaagaccagt | tccttccacagttacgagatga | 167 |
LDHA | caaactgctcatcgtctcaaac | gcaaccacttccaataactctgt | 97 |
LDHB | gcataagatggtggtggacag | atccgagagaggtttttcagc | 120 |
LDHC | tttactgaagggtctggctga | gacgatttttggagtgctgag | 118 |
LDHD | tggcttctgctgtctttccta | gctccctacaactgccttca | 100 |
GAPDH | acagcaacagggtggtggac | tttgagggtgcagcgaactt | 252 |