Table 1 Primer/probe sequences used for determination of nitric oxide synthase isoform expression
From: Upregulation of nitric oxide synthase in mice with severe hypoxia-induced pulmonary hypertension
NOS isoform | Primer/probe |
|---|---|
eNOS | Forward: TCTGCGGCGATGTCACTATG |
| Â | Reverse: CATGCCGCCCTCTGTTG |
| Â | Probe: CCAGCGTCCTGCAAACCGTGC |
iNOS | Forward: TGACGCCAAACATGACTTCAG |
| Â | Reverse: GCCATCGGGCATCTGGTA |
| Â | Probe: AATTCACAGCTCAATCCGGTACGCTGG |
nNOS | Forward: GACTGATGGCAAGCATGACTTC |
| Â | Reverse: GCCCAAGGTAGAGCCATCTG |
| Â | Probe: TGGAACTCGCAGCTCATCCGCTATG |