Figure 1

Dendogram showing the relatedness of gene expression profiles between normal lungs (N), sporadic primary pulmonary hypertension (PPH) lungs, and familial primary pulmonary hypertension (FPPH) lungs. Total RNA from the lung was assayed using Affymetrix HU FL arrays. GeneSpring^® software was used to generate an experimental tree by k-tuple means analysis. The relatedness of each sample to one another is depicted by the dendogram. Blue lines, normal samples; green lines, FPPH samples; and red lines, sporadic PPH. The degree of relatedness is proportional to the length of the lines. Yellow lines, The PPH samples originate from a different phylogeny to the six normal samples or the three FPPH samples, which originate as depicted from the black lines. FPPH refers to a patient whose family history could not be determined, but whose expression pattern suggests a familial form. The black box surrounds a group of genes that appear to be differentially expressed between sporadic PPH and all other samples, and might represent discriminating genes for this condition.