Binding of protegrin-1 to Pseudomonas aeruginosa and Burkholderia cepacia

Respiratory Research

Table 1 C_L and MAC values for various P. aeruginosa and B. cepacia strains determined using the radial diffusion assay.

Organism	Strain	Comments	C_L ^*	MAC^*
P. aeruginosa	144M	Serum sensitive, mucoid, short chain LPS	0.20	0.0625
P. aeruginosa	144M (SR)	Serum resistant, mucoid, long chain LPS	0.18	0.0625
P. aeruginosa	FRD-1	Mucoid strain	0.15	0.125
P. aeruginosa	FRD-2	Nonmucoid strain	0.19	0.125
P. aeruginosa	10145	ATCC strain	0.25	0.125
P. aeruginosa	9027	ATCC strain	0.81	0.50
Mean ± SEM	(all 6 strains)		0.30 ± 0.10^**	0.17 ± 0.07^**
B. cepacia	C6159	Clinical isolate	1.35	6
B. cepacia	C4813	Clinical isolate	5.70	8
B. cepacia	C4878	Clinical isolate	1.53	8
B. cepacia	25416	ATCC strain	7.57	10
B. cepacia	25609	ATCC strain	8.95	10
Mean ± SEM	(all 5 strains)		5.02 ± 1.55^**	8.40 ± 0.75^**

^*Each value represents the mean of three replicate assays, shown in μg/ml. ^**Mann-Whitney test, P. aeruginosa versus B. cepacia, P exact for C_L = 0.004, MAC = 0.004.

ISSN: 1465-993X