Cross-talk between T cells and airway smooth muscle cells in airway responsiveness
- Andrea Heinzmann1
© Biomed Central Ltd 2001
Received: 7 February 2001
Published: 18 September 2001
KeywordsAirway responsiveness, airway smooth muscle cell, T lymphocytes
Over recent years many different cell types have been implicated in the pathophysiology of allergic asthma. Among these, much attention has been focused on the CD4+ T lymphocytes, and their role in the development of asthma has been extensively investigated. The airway smooth muscle (ASM) cell itself has recently been found to have a variety of proinflammatory effects on the atopic asthmatic state. The authors of this study were interested in a possible interaction between resting/activated T cells and resting/activated ASM cells in mediating proasthmatic changes in airway response
In contrast to resting human T cells, anti-CD3-activated T cells significantly enhanced the constrictor response to acetylcholine and impaired the relaxation response to isoproterenol in isolated rabbit ASM tissue. In addition, the incubation of anti-CD3-activated T cells with naive human ASM cells evoked distinctive adhesive clustering. Comparably, exposure of resting T cells to ASM cells prestimulated with IgE immune complexes reciprocally elicited T-cell adhesion. Extended studies showed that the expression of the cell adhesion molecules (CAMs)/costimulatory molecules CD25, CD40, CD40L, CD80, CD86 and CD54, but not CD11a was upregulated by activating naive human ASM cells with anti-CD3-activated T cells. In accordance, resting T cells exposed to activated ASM cells showed a markedly increased expression of the same CAMs/costimulatory molecules. Pretreatment of resting ASM cells with neutralising monoclonal antibodies directed against CD40 and CD86 or against CD11a alone completely abrogated both the activated-T-cell-induced increase in expression of the adhesion molecules and the altered ASM tissue responsiveness to acetylcholine and isoproterenol. Similar effects were seen by pretreating resting T cells with the same mAbs before incubation with activated ASM cells.
The study provides new evidence for the existence of a bidirectional cross-talk between activated T cells and ASM cells. The interaction involves coligation of CAMs/costimulatory molecules and thereby mediates the induction of proasthmatic-like changes in ASM responsiveness. However, the ASM cells used in this study were derived from healthy nonasthmatic humans and rabbits. Thus it still has to be proved whether the same mechanisms are involved in the airway responsiveness of asthmatic humans. Besides, it would be of interest to investigate the release of proinflammatory cytokines during the interaction of these two cell types.
Flow cytometry, RT-PCR, isolation and culture of ASM and T cells, pharmacodynamic studies, microscopy.
- Hakonarson H, Kim C, Whelan R, Campbell D, Grunstein MM: Bi-directional activation between human airway smooth muscle cells and T lymphocytes: role in induction of altered airway responsiveness. J Immunol 2001, 166:293-303. 2001, 166: 293-303.Google Scholar