- Paper Report
- Open Access
Cross-talk between T cells and airway smooth muscle cells in airway responsiveness
- Andrea Heinzmann1
© Biomed Central Ltd 2001
- Received: 7 February 2001
- Published: 18 September 2001
- Airway responsiveness, airway smooth muscle cell, T lymphocytes
Over recent years many different cell types have been implicated in the pathophysiology of allergic asthma. Among these, much attention has been focused on the CD4+ T lymphocytes, and their role in the development of asthma has been extensively investigated. The airway smooth muscle (ASM) cell itself has recently been found to have a variety of proinflammatory effects on the atopic asthmatic state. The authors of this study were interested in a possible interaction between resting/activated T cells and resting/activated ASM cells in mediating proasthmatic changes in airway response
In contrast to resting human T cells, anti-CD3-activated T cells significantly enhanced the constrictor response to acetylcholine and impaired the relaxation response to isoproterenol in isolated rabbit ASM tissue. In addition, the incubation of anti-CD3-activated T cells with naive human ASM cells evoked distinctive adhesive clustering. Comparably, exposure of resting T cells to ASM cells prestimulated with IgE immune complexes reciprocally elicited T-cell adhesion. Extended studies showed that the expression of the cell adhesion molecules (CAMs)/costimulatory molecules CD25, CD40, CD40L, CD80, CD86 and CD54, but not CD11a was upregulated by activating naive human ASM cells with anti-CD3-activated T cells. In accordance, resting T cells exposed to activated ASM cells showed a markedly increased expression of the same CAMs/costimulatory molecules. Pretreatment of resting ASM cells with neutralising monoclonal antibodies directed against CD40 and CD86 or against CD11a alone completely abrogated both the activated-T-cell-induced increase in expression of the adhesion molecules and the altered ASM tissue responsiveness to acetylcholine and isoproterenol. Similar effects were seen by pretreating resting T cells with the same mAbs before incubation with activated ASM cells.
Flow cytometry, RT-PCR, isolation and culture of ASM and T cells, pharmacodynamic studies, microscopy.
- Hakonarson H, Kim C, Whelan R, Campbell D, Grunstein MM: Bi-directional activation between human airway smooth muscle cells and T lymphocytes: role in induction of altered airway responsiveness. J Immunol 2001, 166:293-303. 2001, 166: 293-303.Google Scholar