- Paper Report
- Open Access
Grain-dust-induced airway disease
- Undurti Das1
© Biomed Central Ltd 2001
- Received: 19 March 2001
- Accepted: 18 September 2001
- Published: 18 September 2001
- Critical illness, dopamine, renal failure
Chronic exposure to grain dust can cause irreversible and progressive airway disease. Endotoxin present in organic dust causes airway inflammation. In this study, the authors evaluated the role of endotoxin in the development of chronic dust-induced airway disease using endotoxin-sensitive and hyporesponsive mice.
After an 8 week exposure to corn dust extract and a 4 week recovery period, only the endotoxin-sensitive mice showed significant airway hyperreactivity to subsequent methacholine challenge. A significant increase in the total number of inflammatory cells, tumor necrosis factor-a, interleukin-6, macrophage inflammatory protein-2, and airway modeling, as shown by enhanced deposition of collagen III in the subepithelial area, was noted only in the endotoxin-sensitive mice. These later changes are similar to those seen in symptomatic agricultural workers and asthmatics.
Corn dust extract, endotoxin assay using Limulus amoebocyte lysate assay, lung histochemistry, lung lavage