- Paper Report
- Open Access
Antigen induced NO in asthmatic airways
- Alaina Ammit1
© Biomed Central Ltd 2001
- Received: 26 April 2001
- Published: 14 September 2001
- Antigen, asthma, nitrate, nitrite, NO, S-nitrosothiols
Exhaled nitric oxide (NO) levels are elevated in asthma and other inflammatory lung diseases. Using repeated noninvasive monitoring, it has been suggested that NO is a marker of airway inflammation, but that it may have both anti-inflammatory and proinflammatory effects. The aim of this study was to investigate NO chemical events in the human airway during immediate and late antigen-induced asthmatic responses.
At baseline, and immediately after challenge, NO in asthmatics tended to be higher than in controls. After 48 h NO increased twofold in asthmatics but not in controls. Nitrite levels increased immediately after antigen challenge in asthmatics but not in controls, and had doubled by 48 h. In contrast, nitrate was unaffected by challenge at these time points. Interestingly, at baseline, or immediately after segmental antigen challenge, levels of S-nitrosothiols in asthmatic airways were lower than those found in healthy controls. However, S-nitrosothiol levels increased during the late response to those levels found in healthy controls. The loss of epithelial cells in asthmatic airways hindered accurate quantification of nitrotyrosine in these cells, although a tendency toward more intense staining for nitrotyrosine in the asthmatic epithelium, as compared with the control, was noted. The authors concluded that the dynamics of NO metabolism during an asthmatic response reveal multiple and sequential reactions, suggesting a multifunctional role for NO in the airway.
Segmental antigen challenge, immunohistochemistry, chemiluminescent analyzer, endobronchial biopsies, BAL
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