- Paper Report
- Open Access
PGE2-mediated repair of airway epithelium
- Undurti Das1
© Biomed Central Ltd 2001
- Received: 14 September 2001
- Published: 14 September 2001
- Bronchial asthma, prostaglandins, wound closure
Following inflammatory and physical injury, the airway epithelium needs to be repaired for normal lung function to be restored. Epithelial regeneration involves spreading and migration of cells at the wound edge into the denuded surface to repopulate the wound site. Several factors, such as growth factors, extracellular matrix components and eicosanoids, seem to participate in this airway repair process. Prostaglandins are known to participate in wound healing processes in tissues such as skin, corneal epithelium, and intestinal epithelium. In view of this, the possible role of prostaglandins in regulation of airway epithelium wound closure was examined in this study.
In this in vitro study, the authors found that prostaglandin E2 (PGE2) stimulated wound closure in a dose-dependent fashion. Inhibitors of both cyclo-oxygenase (COX) 1 and 2 which convert arachidonic acid to prostanoids, reduced the rate of wound closure. PGE2 receptors are called E prostanoids 1-4 (EP1-EP4). Using agonists and antagonists, it was observed that PGE2 mediated its stimulatory action on wound healing through EP1 and EP4 but not through the EP3 receptor. Time course studies showed that prostaglandins are critical in the early stages of repair. Metabolites of 5-lipoxygenase did not seem to participate in this wound healing process. This study suggests that PGE2is important for airway epithelial wound healing, and assumes significance in light of the known actions of arachidonic acid and its metabolites in cell protection, growth, angiogenesis and extracellular matrix production.
Human and feline primary tracheal and bronchial epithelial cells, immunoblotting for COX enzymes