Figure 3

Acapsular C. neoformans induces mild LDH release by BEAS-2B cells. BEAS-2B cells were left untreated (NS, white box) or stimulated for 24 h with a MOI = 20 of acapsular C. neoformans grown at RT or 37°C or 50 ng/ml of TNF-α. Supernatants were collected and assayed for LDH release using colorimetry. Data represent the mean ± S.D. of triplicate measurements from two independent experiments and are expressed as a percentage of LDH release from unstimulated BEAS-2B cells treated with a detergent lysis solution (triton).