Figure 6

F508del-CFTR correction in CF-KM4 cells restored local Ca²⁺wave propagation compared to non CF MM39 cells. A Typical traces of Ca²⁺ mobilization in miglustat-treated and untreated CF-KM4 and MM39 during 5 min stimulation by 100 μM histamine in absence of extracellular Ca²⁺. B Histogram of the normalized area under the curve corresponding to the cytoplasmic Ca²⁺ mobilization induced by 100 μM histamine (in 0 mM Ca²⁺) after various treatments. These cells were incubated 2 h at 37°C with 100 μM miglustat (for MM39, CF-KM4 and CF-KM4 reverted cells) or 24 h at 27°C, 100 μM NB-DGJ for CF-KM4 cells. The number on each bar indicates the number of cells. **P < 0.01, *** P < 0.001; ns, non significant difference.