Figure 6

Kinetics in bronchiolar expression of KC and MIP-2 over 24 hrs is different after single vs. repeated CS exposure. Mice were exposed to a single CS exposure (closed circles) or repeatedly exposed to CS for 10 days (open circles). For the 10 day exposure the time point before CS represents 24 hrs after 9 days exposure. At various times up to 24 hrs following last CS exposure, the bronchiolar epithelial cells were harvested by LCM and the expression of KC (A), MIP-2 (B), IL-1β (C), and TNF-α (D) were determined by real-time RT-PCR. Data represent the average expression relative to β2-MG ± SEM from at least six mice. Statistical significance: *** = p < 0.001 vs. before CS exposure at each time point.