Figure 2
From: Bronchiolar chemokine expression is different after single versus repeated cigarette smoke exposure
Initial CS exposure induces oxidative stress in airway epithelial cells. Mice were unexposed (A), exposed to a single CS exposure (B and C), or repeatedly exposed to CS for 10 days (D and E). Lung sections were stained for oxidative DNA stress using an anti-8-OHdG antibody at 1 hr (B and E) or 24 hrs (C and D) following the last CS exposure. Normal mouse IgG1 in place of the 8-OHdG antibody served as a negative control (F). Images are representative of five mice per condition.