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Figure 5 | Respiratory Research

Figure 5

From: NF-kappaB mediates the survival of human bronchial epithelial cells exposed to cigarette smoke extract

Figure 5

Role of p65 in mediating HBEC survival in response to cigarette smoke exposure. Following transfection with p65-siRNA, HBECs were exposed to 10% CSE for 24 hours. Cells were then harvested and used for LIVE/DEAD cytotoxicity/viability assay (Panel A), COMET assay (Panel B) and colony formation assay (Panel C) as described in the methods. Data in panel A and B are one representative from 3 separate experiments, and panel C is an average of 3 separate experiments each performed in triplicate dishes. Panel B: arrow heads indicate cells with DNA damage, and arrows indicated cells undergoing apoptosis.

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Respiratory Research

ISSN: 1465-993X

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