Figure 5

Recruitment of CD8⁺T cells and upregulation of Gr-1 expression during influenza infection. Groups of 5 mice were infected with 10⁵ PFU of HKx31 and at various time points post-infection mice were euthanized and BAL cells were analyzed and quantitated by flow cytometry. (A) Total CD8⁺ T cells and (B) D^bPA₂₂₄-specific CD8⁺ T cells are shown. Data are presented as the mean ± SD cell numbers from the BAL or nasal tissues (NT) of uninfected and virus-infected mice. The expression of Gr-1 was determined on (C) CD8⁺ T cells and (D) D^bPA₂₂₄-specific CD8⁺ T cells recovered by BAL at day 7 post-infection, or on (E) CD8⁺ T cells from the spleen of uninfected mice. Grey histograms represent binding of PE-labelled RB6-8C5 and clear histograms represent binding of an appropriate PE-labelled isotype control. Data shown in (C), (D) and (E) represent a single mouse from a group of 5. All data are representative of 2 or more independent experiments. A minimum of 50,000 living cells (PI^-) were analyzed for each sample.