Figure 5
Inhibition by antileukotrienes of tenascin (Tn) expression in cysteinyl leukotriene-stimulated BEAS-2B human bronchial epithelial cells. A: Flow cytometric analysis showed full inhibition by both montelukast (MLK) and BAY u9773 of LTD4- or LTE4-induced increase in Tn protein expression to the levels not differing from that in non-stimulated cells. Difference from isotype control mean fluorescence intensity (MFI) is presented. B: RT-PCR analysis of Tn mRNA showing inhibition of the cysteinyl leukotriene-induced up-regulation by both MLK and BAY u9773. Partial inhibition of Tn expression induced by combined LTD4 and TGF-β1 down to the level not differing from that what was seen after stimulation with TGF-β1 alone was shown at both protein (A) and mRNA (B) level. There was no statistical difference between the inhibitory effects of MLK and BAY u9773 in any setting. Data are expressed as mean ± SEM (n = 12) *p < 0.05, **p < 0.01, and ***p < 0.001. NS – non-significant.