Figure 4

Results obtained from combining 8 weeks OVA or PBS aerosol exposure with Smoke or Air in sensitized C57BL/6 mice(Combination Protocol 1). (A.) BALF analysis of total cell counts and differentiation after prolonged exposure (8 weeks) to a combination of OVA or PBS aerosols and Smoke or Air. (B) Evaluation of the peribronchial inflammation via a semi-quantitative method (Mean score/airway). (C) Percentage of T – lymphocytes in lung that are CD4⁺CD69⁺ (as a percentage within the T cell population). (D) Percentage of dendritic cells in digested lung tissue (percentage of total lung cells). (E) Th-2 cytokine (IL-13 and TARC) profiles in BALF (pg/ml). (F) Cytokines MCP-1 and KC in BALF (pg/ml). n = 8 mice/group; bars indicate mean ± SEM. * p ≤ 0.05: all groups vs PBS/Air (Mann-Whitney U test); ⁺ p ≤ 0.05: PBS/Smoke vs OVA/Smoke (Mann-Whitney U test); ^# p ≤ 0.05: OVA/Smoke vs OVA/Air (Mann-Whitney U test); ND = Not Detectable (detection limit = 1–2 pg/ml)