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Table 1 Summary of Mouse Clara Cell Isolation Procedures

From: Clara cell adhesion and migration to extracellular matrix

First Author

Date

Mouse Strain

Elutriation

Yield

% Clara

Protease

Ref

Massey, TE

1987

CD1

+

2 × 105

55–65

protease I

[23]

Oreffo, VI

1990

Cba

+

5 × 106

55

trypsin

[21]

Forkert, PG

1990

CD1

+

2 × 105

40

protease I

[33]

Belinsky, SA

1995

A/J

+

ns

76

protease I

[22]

Hynes, DE

1999

CD1

+

ns

64

elastase

[53]

McBride, S

2000

C57Bl/6

+

ns

75

trypsin

[35]

Walker, SR

1989

BDF1

-

4 × 106

88

elastase

[25]

Chichester, CH

1991

Swiss

-

2 × 106

72

elastase

[34]

Corti, M

1996

C57Bl/6

-

ns

75–80

dispase

[28]

Boogaard, PJ

2000

CD1

-

1 × 106

60

trypsin

[24]

Blundell, RA

2005

C3H

-

ns

ns

trypsin

[19]

Atkinson, JJ

2007

Swiss

-

2 × 105

63–80

trypsin

 
  1. ns – not stated