Figure 2

SPLUNC1is not expressed in neutrophils, monocytes or macrophages. Expression of SPLUNC1 was investigated by the use of RT-PCR with exon spanning primer pairs as described in the materials and methods section. Total RNA from B and T cells, neutrophils, monocytes, mononuclear cells and macrophages (either mock treated or infected with Neisseria meningitidis mc58) as well as positive control tissues (nasal epithelium and SPLUNC1-CHO cells), were used as template. The negative controls were a negative reverse transcription reaction performed in the absence of RT enzyme and a PCR reaction performed without Taq polymerase. Primers to the myeloid enriched anti apoptotic gene Bcl2A1 were used as a positive control for all samples.