Typical Densitometry evaluation. Densitometry analysis of immunocytochemistry photographs (n = 5 photos from each sample collected from all mice in each experimental group) for TNF-α, Bax, Bcl-2 Claudin-2, Claudin-4, Claudin-5, ZO-I and β-catenin from lung tissues was assessed. The assay was carried out by using Imaging Densitometer (AxioVision, Zeiss, Milan, Italy) on a personal computer. Data are expressed as % of total tissue area. *P < 0.01 vs. SHAM; °P<0.01 vs. carrageenan- WT group ND: not detectable.