Figure 4
Detection of the VP1 capsid protein in lung tissue. 4a: Positive staining for VP1 protein appears as red intracytoplasmatic coloration: moderate for SO rats (above) and high for 2CLP rats (below). DAPI (blue) was used as nuclear counter-stain. 4b: Negative control for VP1 immunostaining. Above: lung tissue from control septic rats given intratracheal PBS, and below: normal lung tissue from a control T0 rats. The minimal staining seen represents the background. DAPI (blue) was used as nuclear counter-stain. Shown at ×40 magnification.