Figure 2
In vivodetection of luminescence. Detection was performed with a CCCD camera (exposure time 2 min) 48 hours following intratracheal administration of 300 μl of 1.25 × 108 IU/ml SV/luc. 2a: A typical 2CLP rat showing luc activity over right lung and tracheostomy areas (right), left lung and tracheostomy areas (middle), and SO rat showing very low luc activity over pulmonary area (left). The color scales values are indicated on the wedge.2b: Graphic representation of means and standard deviations of 2CLP + SV/luc treated rats. Rt – right lung, Lt – left lung, Trach – tracheostomy area. The values represent the mean for 8 animals and the bars represent standard deviations. The signal over the lung area was different from the signal of tracheal area at P < 0.03 for both left and right lungs.2c: Comparison of SV/luc and Ad/luc transduction (exposure time 2 min) at 48 hours in a rat given intratracheal 300 μl of 1.25 × 108IU/ml SV/luc (left) and a rat given intratracheal 300 μl of 1 × 109 IU/ml Ad/luc (right).2d: Graphic representation of means and standard deviations of 2CLP + SV/luc (8 treated rats, black bar) and 2CLP+Ad/luc (3 treated rats, gray bar).