Figure 8

IL-10 mRNA in HAM stimulated with LPS. Panel A : HAM were preincubated 1 h with inhibitors (25 μM for PD98059, SB203580 and SP600125 or 100 nM for mithramycin) and then stimulated for 24 h with LPS. RNA was extracted as described in details in Materials and Methods. After reverse transcription, specific primers were used to amplify fragment of IL-10 mRNA and the relative abundance of IL-10 compared to β-actin is represented in the graph. Data are mean ± SD from 3 experiments; *p value < 0.05 versus (One sample t test). Panel B: HAM were incubated with LPS (1 μg/ml) for 16 h, translation was stopped using Actinomycin D 2.5 μg/ml and inhibitors (PD98059 or SB203580, 25 μM) were added. IL-10 mRNA was assayed at different time points by real time PCR and corrected for β-actin mRNA.