Figure 7

Localisation of EPC in the left-sided transplanted lung. All pictures show the same lung specimens from rats after left-sided lung transplantation and EPC injection. EPC were stained prior to transplantation with SP-DiIC₁₈(3) (red), followed by staining of histological specimens with the nuclear dye DAPI (blue) and the more endothelial specific FITC-labeled lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) BS-I (green) showing the distribution of EPC. A-C: A-SP-DiIC₁₈(3) (red), B – SP-DiIC₁₈(3) (red) and DAPI (blue), C – merge: SP-DiIC₁₈(3) (red), DAPI (blue) and FITC-labeled lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) BS-I (green) – Orange reveals staining for red and green) [Figure 7C represent magnification and a merge picture of a marked section of Figure 7A, B]; D-F: D: SP-DiIC₁₈(3) (red), E: SP-DiIC₁₈(3) (red) and DAPI (blue), F – merge: SP-DiIC₁₈(3) (red), DAPI (blue) and FITC-labeled lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) BS-I (green) – Orange reveals staining for red and green). F(a): Staining of EPC in culture with DAPI (blue) and FITC-labeled lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) BS-I (green) Abbreviation used: alv stands for alveoli.