Figure 3
From: Reduced inflammatory response in cigarette smoke exposed Mrp1/Mdr1a/1b deficient mice
Histological pictures of paraffin sections of lungs of WT mice (A, C and E) and Mrp1/Mdr1a/1b TKO mice (B, D, and F) that were exposed to cigarette smoke for 6 months. (A, B) H&E staining of lungs of WT and TKO mice. In WT mice there were markedly more inflammatory infiltrates than in lungs of TKO mice. Two types of infiltrates could be distinguished in paraffin sections, infiltrates mainly consisting of pigmented (smoke particles positive) macrophages (m) and infiltrates mainly consisting of lymphoid (ly) cells. (C, D) Lymphoid infiltrates mainly consisted of B-cells (B220 antibody) which were far more present in lungs of WT mice compared to TKO mice, see arrows. (E, F) Pigmented macrophages stained positive with specific antibodies (Mac-3), and were far more present in lungs of WT mice compared to TKO mice, see arrows. Scale bar = 25 μM.