Figure 6

Chemokine expression in AEC II after adoptive CD4⁺T cell transfer into SPC-HA mice. AEC II cells were isolated from the lung of SPC-HA mice one (n = 3), three (n = 3) and six (n = 3) days after adoptive transfer of HA-specific CD4⁺ T cells. Cells were subjected to quantitative real-time RT-PCR analyses. mRNA expression levels of CXCL1, CCL20, CXCL13, CCL11, CXCL2, and RPS9 (as internal control) were analyzed in real-time RT-PCR assays. Relative mRNA amounts were normalized with respect to expression levels in AEC II cells isolated from SPC-HA mice not receiving CD4⁺ T cell transfer (fold change = 1).