Figure 1From: Dietary n-3 fatty acids have suppressive effects on mucin upregulation in mice infected with Pseudomonas aeruginosa Graphical representation of linear regression analyses to validate the quantitative real-time PCR (qPCR) assay. Dilutions of plasmid cDNA were amplified by qPCR in duplicate reactions, and standard curves were created by plotting the cycle threshold (Ct) values versus DNA quantity (Log quantity) in ng for 18s, Muc4, Muc5ac and Muc5b.Back to article page