Figure 7

Light micrographs depict representative histologic fields from the various treatment groups indicated (A-C). A. Annexin V immunostaining is seen in bronchiolar (Br) lining epithelium and associated fibroblast layer (double arrows) and alveolar type II epithelial cells (single arrows). Annexin V is also demonstrated in endothelium in pulmonary vessel (V). Magnification ×300. Bar = 30 μm. B. Annexin V immunostaining from a heparin-treated lung shows similar features to A, but with generally an increase in overall staining, including macrophages (m), not depicted in panel A. Magnification ×300. Bar = 30 μm. C. Histochemical control treated with normal rabbit serum (NRS) in place of rabbit anti-annexin V. Magnification ×200. Bar = 50 μm.