Figure 7
Western blot analyses of Src phosphorylation in A549 cells. A549 cells were treated without (control) or with AYPGKF-NH2 (100 μM) for 10, 30 or 60 min or with thrombin (1 U/ml, 10 min) or with FKGPYA-NH2 (100 μM, 10 min), and then subjected to immunoblotting with specific antibodies for the Y418 phosphorylated Src and total Src protein. (A) Representative Western blot. Stimulation time was 10 min. (B) Summarized densitometric data. Each bar represents the mean ± S.E.M. for 4–6 preparations. **P < 0.01, *P < 0.05 compared with the control.