Figure 5

Effect of actinomycin D on the IL-1β enhanced 5-HT induced contraction. The tracheal segments were cultured for 1 day in the presence of IL-1β (10 ng/ml) with and without actinomycin D (ACD, 5 μg/ml). Each data point is derived from 7–18 experiments and represented as the mean ± S.E.M. Statistical analysis was performed with two-way ANOVA with Bonferroni post-test to compare the two corresponding data points at each concentration of the two curves, no significant differences were seen (P > 0.05).