Figure 3
Receptor characterization. Effect of the selective 5-HT2A receptor antagonist, ketanserin (1–10 nM) on 5-HT induced contraction in the tracheal segments cultured for 4 days with IL-1β (10 ng/ml) (A). Ketanserin was added to the tissue bath 30 min before the first concentration of 5-HT. Each data point is derived from 5–13 experiments and represented as the mean ± S.E.M. Statistical analysis was performed with two-way ANOVA with Bonferroni post-test to compare the two corresponding data points at each concentration of the two curves (Fig. 3A), and unpaired Students' t-test with Welch's correction applied for the comparison of pEC50 values (curve shift) and the Emax (see results). *P < 0.05, compared with control. Schild analysis of data derived from panel A (B).