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Figure 6 | Respiratory Research

Figure 6

From: Cigarette smoke exposure inhibits extracellular MMP-2 (gelatinase A) activity in human lung fibroblasts

Figure 6

Characterisation of MMP-2 forms and complexes in conditioned media. A: identification of zymographic activities in hfl-1 conditioned medium. 1) Zymographic analysis of chromatography-purified conditioned medium of untreated HFL-1 cells; 2) Western blotting of chromatographically-purified HFL-1 conditioned medium with anti-MMP-2 antibody. B: Western blotting with anti-MMP-2 antibody on conditioned media of HFL-1 cells after 24 h treatment. Loading 30 μg/lane.

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