Leukocyte gates on samples from bone marrow, peripheral blood and nasal lavage fluid. Flow cytometry data with dot plots showing gates for neutrophils, basophils, monocytes, lymphocytes, eosinophils and immature granulocytes in bone marrow, peripheral blood and nasal lavage fluid. Immature granulocytes were only found in bone marrow. In nasal lavage fluid only neutrophils could be clearly identified. A) FSC versus SSC with gate R1 representing nucleated leukocytes. B) CD45 versus SSC of cells gated from R1, representing basophils (R5), monocytes (R6) and lymphocytes (R7). C) CD45 versus CD16 of cells gated from R2, representing eosinophils (R8) and immature granulocytes (R9). D) FSC versus CD16 of cells gated from R3, representing neutrophils (R4).