Figure 6

CpG-induced IL-8 is dependent on activation of p38. A. Cells were treated with IL-1β, CpG alone or in combination for 1 h and Western blots were performed for phospho-p38 (top blot) and total p38 (bottom blot). A representative experiment is shown. B. Normalization of five separate experiments (means ± SEM). Compared to control, *p < 0.001; CpG compared to CpG plus IL-1β is not statistically different p = 0.555). C. Cells were pretreated with increasing concentrations ofSB202190 (0.3-3 μM for 1 h) prior to addition of IL-1β or CpG. Cell supernatants were harvested and analyzed for IL-8 by ELISA. Data represent means ± SEM for 3–7 separate experiments (compared to IL-1β alone, *p > 0.001, ANOVA).