Figure 2

CpG synergistically increased IL-1β-induced expression of IL-8. A. 16HBE14o- cells were treated with increasing concentrations of CpG in the absence or presence of IL-1β (0.1 ng/ml). Cell supernatants were harvested and analyzed for IL-8 by ELISA. Data represent means ± SEM for 6 separate experiments (compared to IL-1β alone, *p = 0.001, **p = 0.008, ANOVA). B. Cells were treated with CpG or control CpC in the absence or presence of IL-1β. Data represent means ± SEM for 5 separate experiments (compared to IL-1β alone, *p = 0.038, ANOVA).