Hematoxylin and eosin-stained representative lung sections from the Guinea pig models. Guinea pig lungs sections from sham control (A); lung section after 7 days post-Ad5 infection (Acute model) (B); and after 6 weeks post-Ad5 infection (Chronic model) (C). Arrow indicate regions of inflammation and eosinophilic infiltration, also noted in the Chronic model is the damage to the alveolar parenchyma as indicated by *. Guinea pig airways showing normal histology in Budesonide (Bud) treated lungs (D), whereas ovalbumin (OVA) sensitized/challenged lung sections show airway inflammation and smooth muscle hypertrophy (E). OVA+Bud treated lung section had little eosinophilic infilteration, and no hypertrophy (F). The Ad5+OVA+Bud treated lungs (G) demonstrate damage in the alveolar space, inflammation and alterations of other airway wall components. E1A protein was detected in chronically infected lung sections, arrows indicate positive staining for E1A protein in the nuclei of airway epithelial cells (H) when compared to no staining for isotype control (I). Scale bar represent 100 μm in panels A through G, and 10 μm for panel H and panel I.