Figure 3
Photographs of micro-cytopathic effect neutralization tests. The F(ab')2 against H5N1 virus was diluted into two-fold serial dilutions, and incubated with an equal volume of active H5N1 virus dilution (100 TCID50). After neutralization, each mixture was added to MDCK cell monolayers in micro-plates, and incubated at 37°C to observe CPE status. These photographs showed the morphologic changes of MDCK cells at 72 h after infection. (A) Cell control (no CPE); (B) cell morphologic changes infected with the H5N1 virus; (C) MDCK cells protected from infection of H5N1 virus by anti-H5N1 F(ab')2.