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Figure 3 | Respiratory Research

Figure 3

From: CysLT1 receptor-induced human airway smooth muscle cells proliferation requires ROS generation, EGF receptor transactivation and ERK1/2 phosphorylation

Figure 3

Effect of AG1478 and CysLT 1 -R antagonists on LTD 4 -induced EGF-R phosphorylation. (A) EGF-R phosphorylation induced by 1 μM LTD4 (n = 20) and 0.1 ng/ml EGF (3 minutes) alone and in combination (n = 4). The results are presented as mean ± S.E.M. (B) Representative experiment. (C) EGF-R phosphorylation induced by 1 μM LTD4, 1 ng/ml EGF (3 minutes), in the absence and presence of 250 nM AG1478 (1 h preincubation). D) EGF-R phosphorylation induced by 1 μM LTD4 in the absence and presence of 1 μM of the antagonists zafirlukast and pranlukast (30 minutes pretreatment). 1 ng/ml EGF was used as an internal control. (C-D) The results presented are representative of at least three experiments performed on different cell lines.

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Respiratory Research

ISSN: 1465-993X

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