Figure 2
From: VEGF is upregulated by hypoxia-induced mitogenic factor via the PI-3K/Akt-NF-κB signaling pathway
HIMF induces VEGF expression in mouse lung epithelial cell line. MLE-12 cells were treated with HIMF for various concentrations and periods as indicated. Western blots and semi-quantitative RT-PCR were performed for VEGF expression. (2A) HIMF induced VEGF protein and mRNA production in a dose-dependent manner in MLE-12 cells. (2B) Time-course study indicated that HIMF (40 nmol/L)-induced VEGF production started at 6 h, and persisted for 24 h. Triplicate experiments were performed with essentially identical results.