Figure 4
The ability of ISO to relax an airway is influenced by repetitive stimulation. (A) Relaxation responses of the same contacted airway (with MCh, 200 nM) in response to the first (black dash line) and subsequent 15 minute-exposures of ISO (1 μM) with the time intervals of 10 minutes (light grey line) and 2 hours (dark grey line). The slices were washed with sHBSS between experiments. (B) The summary of the relative relaxation responses induced by repeated ISO stimulation after different intervals. ISO-induced relaxation declined to about half after 10 minutes but was fully recovered after 18 hours. Relaxation was expressed as a percentage of the initial relaxation response. Data from experiments with 5 different airways from 4 mice (*, p < 0.05 compared to the 1st relaxation).