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Table 3 Results of gene expression analysis

From: Lung fibroblasts from patients with emphysema show markers of senescence in vitro

Target gene cDNA array Emphysema/Control qPCR Controls qPCR Emphysema p-value
IGFBP-3 1.3 0.38 (0.15; 0.40) 0.82 (0.66; 1.36) 0.024*
IGFBP-5 0.19 115.0 (60.5; 161.0) 73.6 (49.0; 159.0) 0.51
IGFBP-rP1 (IGFBP-7) 4.87 4.25 (2.67; 5.59) 9.6 (6.77; 12.4) 0.002**
IGFBP-rP2 (CTGF) n.d. 2.97 (1.68; 4.25) 4.40 (3.86; 4.99) 0.070
IGFBP-rP4 (Cyr61) 0.84 258 (222; 329) 198 (185; 322) 0.57
LOXL2 3.88 1.27 (0.73; 1.79) 1.41 (1.22; 1.92) 0.63
OAZ1 0.15 0.22 (0.17; 0.25) 0.18 (0.18; 0.25) 0.96
FOSL1 0.21 0.89 (0.63; 1.16) 1.00 (0.78; 1.34) 0.57
CDK4 0.16 0.91 (0.58; 1.18) 0.73 (0.53; 0.78) 0.63
  1. The cDNA array column gives the differential gene expression in the exploratory 12 k array experiment as ratio of emphysema to control. qPCR columns give the gene expression of the target gene relative to PBGD for control patients and patients with emphysema. These values are the median values (IQR) of the normalized ratios as derived from RelQuant software. They were additionally corrected by using ratios of qPCR calibrators. P-values refer to the comparison of qPCR results between the two groups and were calculated by the Mann-Whitney U-test. *p < 0.05, **p < 0.01, n.d. not determined.