Bronchoalveolar lavage tryptase levels. Tryptase levels were determined by ELISA in the bronchoalveolar lavage fluid harvested at 48 hours after the last intratracheal challenge. C5 deficient mice had significantly higher levels of then these C5 sufficient mice. Values represent mean ± SEM with n = 11–15 for each group. **p < 0.01 compared to C5 Sufficient mice.