Figure 3

Reduced SOC activated inward current in STIM1 suppressed cells compared with control and STIM2 suppressed cells assessed by whole cell patch clamp. (a) A time course of current density (measured at -100 mV), each point represents mean data ± sem of all cells in each group; control cells treated with scrambled siRNA (black circles, n = 16), STIM1 suppressed cells (blue squares, n = 10) and STIM2 suppressed cells (red triangles, n = 12). (b) Current-voltage (I-V) relationships at the steady-state SOC current (ie addition of 10 μM CPA in nominally Ca²⁺ free buffer) and represents averaged data from all cells of each experimental group. (c) A bar chart illustrating peak CPA sensitive current density (measured at -100 mV) of cells treated with scrambled control, STIM-1 or STIM-2 siRNA. Data are indicated as statistically significant with **P < 0.01.