Table 1 Oligonucleotide primers for cav-1, cav-2 and β-MG (β-2-microglobulin) in RT-PCR analysis
From: Caveolin-1 and -2 in airway epithelium: expression and in situ association as detected by FRET-CLSM
Gene | GenBank accession NO | Primer | Product length (bp) | Position of amplified DNA (bp) |
|---|---|---|---|---|
cav-1 | Z46614.1 | Forward: CAGCATGTCTGGGGGTAAAT Reverse: TGCTTCTCATTCACCTCGTCT | 123 | 25–147 |
cav-1 | Z46614.1 | Forward: GGCTAGCTTCACCACCTTCA Reverse: GTGCAGGAAAGAGAGGATGG | 121 | 165–285 |
cav-2 | BC062059.1 | Forward: TGTTTCTAGCCATCCCCTTG Reverse: ACCATGAGGCAGGTCTTCAC | 106 | 392–497 |
cav-2 | BC062059.1 | Forward: CCTACAGCCACCACAGTGTC Reverse: GGTTCTGCGATCAGATCCTC | 127 | 176–302 |
β-MG | NM_012512 | Forward: TGTCTCAGTTCCACCCACCT Reverse: GGGCTCCTTCAGAGTGACG | 191 | 147–337 |