RT-PCR, rat. (A) Cav-1 and cav-2 mRNAs were detected in abraded tracheal epithelium (TE) and in microdissected (picked) tracheal epithelial cells (TEC) with primer sets spanning 25–147 bp and 392–497 bp within the coding region, respectively. Control reactions for each primer pair included the absence of template (H20), absence of reverse transcriptase (Ø RT), and analysis of samples of dried O.C.T. compound that were located adjacent to the luminal side of the collected tracheal epithelial cells. (B) Real-time RT-PCR. CT values for β-MG, cav-1 and cav-2 derived from cDNA from abraded tracheal epithelial cells; n = number of animals.