Figure 1

The effect of the iron chelating agent deferoxamine (DFO) on CP^•nitroxide signal intensity in vitro. (A) Typical ESR spectrum of CP^• nitroxide resulting from the reaction of the hydroxylamine spin probe CPH with ROS. The height of the first field component of the triple-line spectrum was used for quantification of signal intensity. (B) In-vitro incubation of CPH (1 mM) in Krebs-Henseleit buffer. Signal intensity is given in arbitrary units (AU). Data are shown for CPH oxidation in the absence (-DFO) or in the presence of either 20 μM or 2 mM deferoxamine (DFO). Asterisks indicate significant differences when compared to the-DFO group.