Figure 4

Children with pulmonary alveolar proteinosis. Western blotting of a lavage from patient PAP 12 (only NFPROX bands) and PAP 04 (all other bands) to demonstrate the most frequent abnormalities. After SDS-PAGE and transfer, the membranes were probed with the antibodies indicated. The pro-forms were probed in the absence (-) and presence (+) of an excess of the peptide used to raise this antibody. Note that bands that are not displaced by the competing peptide were not considered as specific bands and they are marked by an asterisk. The numbers next to the lanes indicate the molecular weights in kDa. The arrowheads indicate the abundance of SP-B, the bands at 19–21 and 25–26 kDa using CTERMB which also react with NFLANK, and some of the break-down fragments reacting with NFPROX which are more frequently seen in this condition and in cRD as compared to the other lung diseases (see figure 5). All bands were analyzed under reducing conditions.