Figure 1

mRNA expression of CCL2 and CXCR3 agonistic chemokines by A549 cells. A : Dose response of proinflammatory cytokine-induced CCL2, CXCL10, CXCL11, and CXCL9 mRNA accumulation. The representative gel images from one out of three independent experiments are shown. Expression of β-actin in the same samples demonstrates equal loading of lanes. B : Densitometric analysis of the CCL2, CXCL10, CXCL11, and CXCL9 mRNA expression. RT-PCR was performed with total RNA obtained from A549 cells stimulated for the indicated times with 50 U/ml IFN-γ (IFN50), 50 U/ml IFN-γ and 5 ng/ml TNF-α (IFN50+TNF5) or 50 U/ml IL-1β (IFN50+IL50), and a combination of cytokines (CTMX) 50 U/ml of IFN-γ and IL-1β, and 5 ng/ml TNF-α. The mRNA-amplificates from each culture was quantitated individually. The distinct dots on the lines represent the mean percentages of β-actin density of duplicate determinations at each individual time-point for different concentrations/combinations of cytokines. Data are from one representative experiment out of three. C : Dose- and time-dependent effects of TNF-α, IFN-γ, and IL-1β at indicated concentrations on CXCL11 mRNA expression by A549 cells are shown.