Figure 4

Irrespective of infection, CD11c⁺T cells are recruited to the lung more effectively that to the spleen. Lungs were harvested on day 8 after RSV infection; CD8⁺ cells were isolated by magnetic cell sorting and labeled with CFSE. Subsequently cells were adoptively transferred by intravenous injection into naive or RSV infected mice. 3 hours after transfer lungs and spleens of recipient mice were harvested and expression of CD11c on transferred cells was analyzed by FACS. A) Dot plots show gating of transferred CFSE⁺CD8⁺ cells and staining of gated cells with isotype control or anti-CD11c antibody. B) The bars indicate percentage of transferred CSFE⁺ CD8⁺ cells staining positive for CD11c in lungs and spleens after pre-incubation of transferred cells with isotype control (open bars) or anti-CD11c-mAb (hatched bars) (mean ± SD, n = 4, * significant difference, p < 0.05, lung naïve and lung RSV versus spleen naïve and spleen RSV).