Figure 2

Differential expression of surface markers on pulmonary CD11c⁺and CD11c^-CD8⁺T cells. Lungs of naive mice or on day 8 after RSV infection were harvested. Cells were stained with antibodies for CD8, CD11c, and the surface markers indicated. A) Dot plots show expression of CD11c versus the indicated marker on cells gated for lymphocyte size and CD8 expression after RSV infection. Numbers indicate the percentage of cells in corresponding quadrants. Quadrants were set according to isotype controls except for CD11a, CD54 and CD62L. Since these three molecules are expressed to a considerable degree on naïve CD8 T cells, this expression was used for quadrant settings to highlight differences in CD11⁺ CTL. B) Histograms show expression of CD54, CD62L, and CD69 on pulmonary CD8⁺ cells from naïve (dotted line) or RSV infected mice (CD11c⁺ cells: black line, CD11c^- cells: filled histogram).